ARHGDIB monoclonal antibody, clone 97A1015
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ARHGDIB in Jurkat cells using ARHGDIB monoclonal antibody, clone 10D774 (Cat # MAB0058) at 2 ug/mL and ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) at 0.1 ug/mL. Cells were treated with 2 uM staurosporine for different time periods. ARHGDIB monoclonal antibody, clone 10D774 (Cat # MAB0058) detects both the full-length and cleaved forms of ARHGDIB while ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) specifically detects only the cleaved form of the protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) (bottom) at 5 ug/mL .
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ARHGDIB.
Immunogen
A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human ARHGDIB.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2b
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ARHGDIB in Jurkat cells using ARHGDIB monoclonal antibody, clone 10D774 (Cat # MAB0058) at 2 ug/mL and ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) at 0.1 ug/mL. Cells were treated with 2 uM staurosporine for different time periods. ARHGDIB monoclonal antibody, clone 10D774 (Cat # MAB0058) detects both the full-length and cleaved forms of ARHGDIB while ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) specifically detects only the cleaved form of the protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and ARHGDIB monoclonal antibody, clone 97A1015 (Cat # MAB0059) (bottom) at 5 ug/mL .Immunoprecipitation
Flow Cytometry
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Gene Info — ARHGDIB
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Interactome
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Pathway
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Disease
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Publication Reference
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Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL.
Jenkins CE, Swiatoniowski A, Power MR, Lin TJ.
Journal of Immunology 2006 Dec; 177(11):8000.
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Pseudomonas aeruginosa exotoxin A induces human mast cell apoptosis by a caspase-8 and -3-dependent mechanism.
Jenkins CE, Swiatoniowski A, Issekutz AC, Lin TJ.
The Journal of Biological Chemistry 2004 Jun; 279(35):37201.
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Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Liu CY, Lee CF, Hong CH, Wei YH.
Annals of the New York Academy of Sciences 2004 Apr; 1011:133.
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Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species.
Ko CH, Shen SC, Chen YC.
Free Radical Biology & Medicine 2004 Apr; 36(7):897.
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Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids.
Yen-Chou Chen, Shing-Chuan Shen, Hui-Yi Lin.
Biochemical Pharmacology 2003 Oct; 66(7):1139.
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Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade.
Shing-Chuan Shen, Yen-Chou Chen, Feng-Lin Hsu, Woan-Rouh Lee.
Journal of Cellular Biochemistry 2003 Aug; 89(5):1044.
Application：WB-Ce, Human, HL-60, THP-1 cells.
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Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
Yen-Chou Chen, Shing-Chuan Shen, Woan-Ruoh Lee, Foun-Lin Hsu, Hui-Yi Lin, Ching-Huai Ko, Shi-Wen Tseng.
Biochemical Pharmacology 2002 Dec; 64(12):1713.
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RhoGDI2 is an invasion and metastasis suppressor gene in human cancer.
John J Gildea, M Jabed Seraj, Gary Oxford, Michael A Harding, Garret M Hampton, Christopher A Moskaluk, Henry F Frierson, Mark R Conaway, Dan Theodorescu.
Cancer Research 2002 Nov; 62(22):6418.
Application：WB-Ce, WB-Tr, Human, Jurkat, T24 cells.
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Interference with PDK1-Akt survival signaling pathway by UCN-01 (7-hydroxystaurosporine).
Saori Sato, Naoya Fujita, Takashi Tsuruo.
Oncogene 2002 Mar; 21(11):1727.
Application：WB-Ce, Human, HEK 293T, HT-1080 cells.
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Epothilone B analogue (BMS-247550)-mediated cytotoxicity through induction of Bax conformational change in human breast cancer cells.
Hirohito Yamaguchi, Shanthi R Paranawithana, Michael W Lee, Ziwei Huang, Kapil N Bhalla, Hong-Gang Wang.
Cancer Research 2002 Jan; 62(2):466.
Application：WB-Ce, Human, MDA-MB-468 cells.
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The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change.
Yamaguchi H, Wang HG.
Oncogene 2001 Nov; 20(53):7779.
Application：WB-Tr, Mouse, FL5.12 cells.
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Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Cuddeback SM, Yamaguchi H, Komatsu K, Miyashita T, Yamada M, Wu C, Singh S, Wang HG.
The Journal of Biological Chemistry 2001 Jun; 276(23):20559.
Application：WB-Ce, Human, FL5.12 cells.
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Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL.
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