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Last updated: 2019/8/18
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His tag monoclonal antibody, clone H21-5 

  • Catalog # : MAB0041
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant protein with His tag.
  • Immunogen:
  • Polypeptide containing the N-terminus 6 x His tag.
  • Host:
  • Mouse
  • Specificity:
  • This antibody has been selected for its ability to detect proteins containing consecutive histidine regions. The antibody detects polyhistidine tags localized at the N-terminus.
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody was produced from a mouse hybridoma (clone# H21-5) elicited from a mouse immunized with a polypeptide containing the N-terminus 6 x histidine tag. The lgG fraction of ascites fluid was purified by Protein G affinity chromatography.
  • Isotype:
  • IgG3, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein with His tag on ELISA and Western Blot and Affinity chromatography.
  • Recommend Usage:
  • Affinity chromatography
    ELISA
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS.
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    Keep the vial on ice prior to restoration. For preparing a 1 mg/mL stock solution, reconstitute the antibody by addition of 100 uL of 50% glycerol, mix gently and let it stand for 5 min on ice. Using deionized water instead of 50% glycerol may be good, but for extended storage, 50% glycerol is recommended. For an optimal storage, aliquot into small portions and store at -20°C or below. Avoid repeated freeze-thaw cycles.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Recombinant protein)
  • ELISA
  • Affinity Chromatography
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Recombinant protein)
  • ELISA
  • Affinity Chromatography
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