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Last updated: 2019/12/8
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Histamine monoclonal antibody, clone HYHIS1 

  • Catalog # : MAB0021
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against conjugated histamine.
  • Immunogen:
  • Synthetic histamine conjugated to bovine serum albumin (BSA).
  • Host:
  • Mouse
  • Specificity:
  • Using Histamine-BSA conjugate (Hist-BSA), antibody specificity was performed by using an ELISA test by competition experiments:
    Histamine-BSA: Kd around 10-8 M; very high affinity and specificity
    No crossreactivity with close competitors:
    Competitors (Affinity compared to Hist-BSA)
    1-Methyl-Histamine-BSA (0 time lower; high affinity)
    Histidine-BSA (50000 times; lower no crossreactivity)
    The antibody recognizes the conjugated form of the small molecule, not the free form.
  • Form:
  • Lyophilized
  • Purification:
  • Ammonium sulfate precipitation
  • Isotype:
  • IgG, kappa
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Immunohistochemistry (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule.
  • Applications
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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