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Last updated: 2016/10/23
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PPP1R14A (phospho T38) monoclonal antibody, clone AK-1F11

  • Catalog # : MAB0013
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of PPP1R14A.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T38 of human PPP1R14A.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Phospho-CPI-17 Thr38 Antibody detects phosphorylated recombinant CPI-17 when phosphorylated at threonine38 in vitro by means of western blotting and ELISA.
  • Recommend Usage:
  • Western Blot (1-2 ug/mL)
    ELISA (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • In vitro kinase reaction and detection of phosphorylation of CPI-17 at Thr38 residue by using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013). GST-CPI-17 was phosphorylated by protein kinase C derived from rat brain in vitro and proved by CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013) after SDS-PAGE and transfer to PVDF membrane.
  • ELISA for measurement of recombinant PKC activity using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 (Cat # MAB0013).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Gene Name:
  • PPP1R14A
  • Gene Alias:
  • CPI-17,CPI17,PPP1INL
  • Gene Description:
  • protein phosphatase 1, regulatory (inhibitor) subunit 14A
  • Gene Summary:
  • PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM
  • Other Designations:
  • 17-KDa protein,17-kDa PKC-potentiated inhibitory protein of PP1,PKC-potentiated inhibitory protein of PP1
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