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Last updated: 2017/7/23
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CDC25C (phospho S216) monoclonal antibody, clone TK-1F1

  • Catalog # : MAB0007
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of CDC25C.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S216 of human CDC25C.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Phospho-Cdc25C Ser216 Antibody detects phosphorylated recombinant Cdc25C only when phosphorylated at serine216 in vitro, by western blotting.
  • Recommend Usage:
  • Western Blot (1-2 ug/mL)
    ELISA (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • In vitro kinase reaction and detection of phosphorylation of Cdc25C at Ser216 residue by using Cdc25C (phospho S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007). GST-Cdc25C was phosphorylated by Checkpoint kinase like as Chk1 and proved by Cdc25C (phospho-S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007) after SDS-PAGE and transfer to PVDF membrane.
  • ELISA
  • ELISA
  • ELISA for measurement of recombinant Chk1 and Chk2 activity using Cdc25C (phospho-S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 995
  • Gene Name:
  • CDC25C
  • Gene Alias:
  • CDC25
  • Gene Description:
  • cell division cycle 25 homolog C (S. pombe)
  • Gene Summary:
  • This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq
  • Other Designations:
  • cell division cycle 25C,cell division cycle 25C protein,dual specificity phosphatase CDC25C,m-phase inducer phosphatase 3,mitosis inducer CDC25,phosphotyrosine phosphatase
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