In vitro kinase reaction and detection of phosphorylation of Cdc25C at Ser216 residue by using Cdc25C (phospho S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007). GST-Cdc25C was phosphorylated by Checkpoint kinase like as Chk1 and proved by Cdc25C (phospho-S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007) after SDS-PAGE and transfer to PVDF membrane.
ELISA for measurement of recombinant Chk1 and Chk2 activity using Cdc25C (phospho-S216) monoclonal antibody, clone TK-1F1 (Cat # MAB0007).
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq