RB1 (phospho S807) monoclonal antibody, clone 5H12
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More Files
- More Functions
Western Blot (Tissue lysate)
RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005). Western blot analysis of pRb in human stomach.
Western Blot (Cell lysate)
Western blot analysis of in extracts from MOLT 4 cell using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).
Immunoprecipitation
IP-Western in HL-60 cell lysate using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of recombinant Cdk4-CyclinD kinase activity using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of Rb.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S807 of human Rb.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Quality Control Testing
Phospho-Rb Ser807 Antibody detects endogenous Rb protein only when phosphorylated at Serine 807.
Recommend Usage
Western Blot (1-2 ug/mL)
Immunoprecipitation (2-4 ug/mL)
ELISA (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005). Western blot analysis of pRb in human stomach.Western Blot (Cell lysate)
Western blot analysis of in extracts from MOLT 4 cell using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).Immunoprecipitation
IP-Western in HL-60 cell lysate using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).Enzyme-linked Immunoabsorbent Assay
ELISA measurement of recombinant Cdk4-CyclinD kinase activity using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005). -
Gene Info — RB1
Entrez GeneID
5925GeneBank Accession#
NM_000321Protein Accession#
NP_000312Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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