RB1 (phospho S612) monoclonal antibody, clone 3C11
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More Files
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Western Blot (Cell lysate)
RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003). Western blot analysis of pRb in HeLa.
Western Blot (Cell lysate)
Western blot analysis in extracts from MOLT 4 cell using RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003).
Immunoprecipitation
IP-Western blotting in HL-60 cell lysate using RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003 ; Lane 2).
Enzyme-linked Immunoabsorbent Assay
ELISA for measurement of recombinant Cdc2-CyclinB1 kinase activity using RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of Rb.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S612 of human Rb.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Quality Control Testing
Phospho-Rb Ser612 Antibody detects endogenous Rb protein only when phosphorylated at serine612.
Recommend Usage
Western Blot (1-2 ug/mL)
Immunoprecipitation (2-4 ug/mL)
ELISA (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003). Western blot analysis of pRb in HeLa.Western Blot (Cell lysate)
Western blot analysis in extracts from MOLT 4 cell using RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003).Immunoprecipitation
IP-Western blotting in HL-60 cell lysate using RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003 ; Lane 2).Enzyme-linked Immunoabsorbent Assay
ELISA for measurement of recombinant Cdc2-CyclinB1 kinase activity using RB1 (phospho S612) monoclonal antibody, clone 3C11 (Cat # MAB0003). -
Gene Info — RB1
Entrez GeneID
5925GeneBank Accession#
NM_000321Protein Accession#
NP_000312Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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