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Last updated: 2017/7/23
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MBS/MYPT1 (phospho T696) monoclonal antibody, clone AF-20

  • Catalog # : MAB0001
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of MBS/MYPT1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T696 of human MBS/MYPT1.
  • Host:
  • Mouse
  • Reactivity:
  • Chicken, Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Phospho-MBS/MYPT1 Thr696 Antibody detects endogenous MBS/MYPT1 only when phosphorylated at threonine696. The antibody does not recognize other myosin phosphatase regulatory subunit.
  • Recommend Usage:
  • Western Blot (1-2 ug/mL)
    ELISA (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunofluorescence
  • ELISA
  • ELISA
  • ELISA for measurement of recombinant Rho-kinase activity using MBS/MYPT1 (phospho T696) monoclonal antibody, clone AF-20 (Cat # MAB0001).
  • Application Image
  • Western Blot
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 4659
  • Gene Name:
  • PPP1R12A
  • Gene Alias:
  • MBS,MGC133042,MYPT1
  • Gene Description:
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
  • Gene Summary:
  • Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • myosin phosphatase, target subunit 1
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