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Last updated: 2017/7/23

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293T whole cell lysate (denatured)

  • Catalog # : L151V1
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  • Specification
  • Product Description:
  • Whole cell lysate (denatured)
  • Tissue:
  • Kidney
  • Host:
  • Human
  • Preparation Method:
  • Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.
  • Lysis Buffer:
  • 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
  • Quality Control Testing:
  • 12.5% SDS-PAGE Stained with Coomassie Blue.
    SDS-PAGE Gel
    QC Testing of L151V1
  • Recommend Usage:
  • The lysate is ready to load on SDS-PAGE for Western blotting.
  • Storage Buffer:
  • In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
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