293T whole cell lysate (denatured)
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Specification
Product Description
Whole cell lysate (denatured)
Tissue
Kidney
Host
Human
Preparation Method
Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.
Lysis Buffer
1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE Gel
Recommend Usage
The lysate is ready to load on SDS-PAGE for Western blotting.
Storage Buffer
In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Publication Reference
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Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells.
Kokhaei P, Hojjat-Farsangi M, Mozaffari F, Moshfegh A, Pak F, Rashidy-Pour A, Palma M, Hansson L, Osterborg A, Mellstedt H.
PLoS One 2018 Feb; 13:1.
Application:qRT-PCR, Human, 293T cells.
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Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells.
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