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CEBPG HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L150T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CEBPG full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 16
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CEBPG antibody (H00001054-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L150T6
    CEBPG transfected lysate
    Western Blot
    QC Testing of L150T6
    Lane 1: CEBPG transfected lysate ( 16 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1054
  • Gene Name:
  • CEBPG
  • Gene Alias:
  • GPE1BP,IG/EBP-1
  • Gene Description:
  • CCAAT/enhancer binding protein (C/EBP), gamma
  • Gene Summary:
  • The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. [provided by RefSeq
  • Other Designations:
  • CCAAT/enhancer binding protein gamma
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