BIRC4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-BIRC4 full length
Host
Human
Theoretical MW (kDa)
57
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BIRC4 antibody (H00000331-M01) by Western Blots.
SDS-PAGE Gel
BIRC4 transfected lysate
Western Blot
Lane 1: BIRC4 transfected lysate ( 57 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — XIAP
Entrez GeneID
331GeneBank Accession#
BC032729Protein Accession#
AAH32729Gene Name
XIAP
Gene Alias
API3, BIRC4, ILP1, MIHA, XLP2
Gene Description
X-linked inhibitor of apoptosis
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq
Other Designations
OTTHUMP00000023975|OTTHUMP00000196392|apoptosis inhibitor 3|baculoviral IAP repeat-containing 4|baculoviral IAP repeat-containing protein 4
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Interactome
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Pathway
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Disease
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