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BCAR3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L147T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-BCAR3 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 93
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BCAR3 antibody (H00008412-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L147T6
    BCAR3 transfected lysate
    Western Blot
    QC Testing of L147T6
    Lane 1: BCAR3 transfected lysate ( 93 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 8412
  • Gene Name:
  • BCAR3
  • Gene Alias:
  • KIAA0554,NSP2,SH2D3B
  • Gene Description:
  • breast cancer anti-estrogen resistance 3
  • Gene Summary:
  • Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000011959,OTTHUMP00000011960,OTTHUMP00000011961,breast cancer antiestrogen resistance 3,dJ1033H22.2 (breast cancer anti-estrogen resistance 3)
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