BCAR3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-BCAR3 full length
Host
Human
Theoretical MW (kDa)
93
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BCAR3 antibody (H00008412-M01) by Western Blots.
SDS-PAGE Gel
BCAR3 transfected lysate
Western Blot
Lane 1: BCAR3 transfected lysate ( 93 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — BCAR3
Entrez GeneID
8412GeneBank Accession#
BC039895Protein Accession#
AAH39895Gene Name
BCAR3
Gene Alias
KIAA0554, NSP2, SH2D3B
Gene Description
breast cancer anti-estrogen resistance 3
Omim ID
604704Gene Ontology
HyperlinkGene Summary
Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. [provided by RefSeq
Other Designations
OTTHUMP00000011959|OTTHUMP00000011960|OTTHUMP00000011961|breast cancer antiestrogen resistance 3|dJ1033H22.2 (breast cancer anti-estrogen resistance 3)
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Interactome
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