Transient overexpression cell lysate was tested with Anti-AKR1C2 antibody (H00001646-M03 ) by Western Blots. SDS-PAGE Gel
AKR1C2 transfected lysate Western Blot
Lane 1: AKR1C2 transfected lysate ( 37 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq
OTTHUMP00000018995,OTTHUMP00000044759,aldo-keto reductase family 1, member C2,chlordecone reductase homolog,pseudo-chlordecone reductase,trans-1,2-dihydrobenzene-1,2-diol dehydrogenase,type II dihydrodiol dehydrogenase