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AKR1C2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L145T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-AKR1C2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 37
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-AKR1C2 antibody (H00001646-M03 ) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L145T6
    AKR1C2 transfected lysate
    Western Blot
    QC Testing of L145T6
    Lane 1: AKR1C2 transfected lysate ( 37 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1646
  • Gene Name:
  • AKR1C2
  • Gene Alias:
  • AKR1C-pseudo,BABP,DD,DD2,DDH2,HAKRD,HBAB,MCDR2
  • Gene Description:
  • aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
  • Gene Summary:
  • This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000018995,OTTHUMP00000044759,aldo-keto reductase family 1, member C2,chlordecone reductase homolog,pseudo-chlordecone reductase,trans-1,2-dihydrobenzene-1,2-diol dehydrogenase,type II dihydrodiol dehydrogenase
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