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AKAP10 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L144T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-AKAP10 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 74
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-AKAP10 antibody (H00011216-M04 ) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L144T6
    AKAP10 transfected lysate
    Western Blot
    QC Testing of L144T6
    Lane 1: AKAP10 transfected lysate ( 74 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • AKAP10
  • Gene Alias:
  • D-AKAP2,MGC9414,PRKA10
  • Gene Description:
  • A kinase (PRKA) anchor protein 10
  • Gene Summary:
  • The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq
  • Other Designations:
  • A-kinase anchor protein 10,dual-specificity A-kinase anchoring protein 2,mitochondrial A kinase PPKA anchor protein 10,protein kinase A anchoring protein 10
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