AKAP10 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
- More Functions
SDS-PAGE Gel
AKAP10 transfected lysate
Western Blot
Lane 1: AKAP10 transfected lysate ( 74 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-AKAP10 full length
Host
Human
Theoretical MW (kDa)
74
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AKAP10 antibody (H00011216-M04 ) by Western Blots.
SDS-PAGE Gel
AKAP10 transfected lysate
Western Blot
Lane 1: AKAP10 transfected lysate ( 74 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — AKAP10
Entrez GeneID
11216GeneBank Accession#
NM_007202Protein Accession#
NP_009133Gene Name
AKAP10
Gene Alias
D-AKAP2, MGC9414, PRKA10
Gene Description
A kinase (PRKA) anchor protein 10
Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq
Other Designations
A-kinase anchor protein 10|dual-specificity A-kinase anchoring protein 2|mitochondrial A kinase PPKA anchor protein 10|protein kinase A anchoring protein 10
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Interactome
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Disease
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