Transient overexpression cell lysate was tested with Anti-SFRP4 antibody (H00006424-A01) by Western Blots. SDS-PAGE Gel
SFRP4 transfected lysate Western Blot
Lane 1: SFRP4 transfected lysate ( 40 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq
secreted frizzled-related protein 4,secreted frizzled-related protein 4; secreted frizzled-related protein 4