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SFRP4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L139T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-SFRP4 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 40
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SFRP4 antibody (H00006424-A01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L139T6
    SFRP4 transfected lysate
    Western Blot
    QC Testing of L139T6
    Lane 1: SFRP4 transfected lysate ( 40 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 6424
  • Gene Name:
  • SFRP4
  • Gene Alias:
  • FRP-4,FRPHE,MGC26498
  • Gene Description:
  • secreted frizzled-related protein 4
  • Gene Summary:
  • Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq
  • Other Designations:
  • secreted frizzled-related protein 4,secreted frizzled-related protein 4; secreted frizzled-related protein 4
  • Gene Pathway
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