MAGEA2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-MAGEA2 full length
Host
Human
Theoretical MW (kDa)
35
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MAGEA2 antibody (H00004101-M01) by Western Blots.
SDS-PAGE Gel
MAGEA2 transfected lysate
Western Blot
Lane 1: MAGEA2 transfected lysate ( 35 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — MAGEA2
Entrez GeneID
4101GeneBank Accession#
BC063681Protein Accession#
AAH63681Gene Name
MAGEA2
Gene Alias
MAGE2, MAGEA2A, MGC131923
Gene Description
melanoma antigen family A, 2
Omim ID
300173Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000024219|melanoma antigen 2|melanoma-associated antigen 2
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