LLGL2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-LLGL2 full length
Host
Human
Theoretical MW (kDa)
113
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LLGL2 antibody (H00003993-M06) by Western Blots.
SDS-PAGE Gel
LLGL2 transfected lysate
Western Blot
Lane 1: LLGL2 transfected lysate ( 113 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — LLGL2
Entrez GeneID
3993GeneBank Accession#
NM_004524Protein Accession#
NP_004515Gene Name
LLGL2
Gene Alias
HGL, LGL2
Gene Description
lethal giant larvae homolog 2 (Drosophila)
Gene Ontology
HyperlinkGene Summary
The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
human giant larvae homolog|lethal giant larvae homolog 2
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Interactome
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Pathway
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Disease
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