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LLGL2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L137T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-LLGL2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 113
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-LLGL2 antibody (H00003993-M06) by Western Blots.
    Western Blot
    QC Testing of L137T6
    Lane 1: LLGL2 transfected lysate ( 113 KDa).
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of L137T6
    LLGL2 transfected lysate
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3993
  • Gene Name:
  • LLGL2
  • Gene Alias:
  • HGL,LGL2
  • Gene Description:
  • lethal giant larvae homolog 2 (Drosophila)
  • Gene Summary:
  • The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • human giant larvae homolog,lethal giant larvae homolog 2
  • Gene Pathway
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