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ILK HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L134T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ILK full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 51.4
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ILK antibody (H00003611-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L134T6
    ILK transfected lysate
    Western Blot
    QC Testing of L134T6
    Lane 1: ILK transfected lysate ( 51.4 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3611
  • Gene Name:
  • ILK
  • Gene Alias:
  • DKFZp686F1765,P59
  • Gene Description:
  • integrin-linked kinase
  • Gene Summary:
  • Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
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