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HOXB7 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L133T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-HOXB7 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 24
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HOXB7 antibody (H00003217-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L133T6
    HOXB7 transfected lysate
    Western Blot
    QC Testing of L133T6
    Lane 1: HOXB7 transfected lysate ( 24 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3217
  • Gene Name:
  • HOXB7
  • Gene Alias:
  • HHO.C1,HOX2,HOX2C,Hox-2.3
  • Gene Description:
  • homeobox B7
  • Gene Summary:
  • This gene is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded nuclear protein functions as a sequence-specific transcription factor that is involved in cell proliferation and differentiation. Increased expression of this gene is associated with some cases of melanoma and ovarian carcinoma. [provided by RefSeq
  • Other Designations:
  • homeo box 2C,homeo box B7,homeo box c1 protein
  • Related Disease
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