Product Browser

Last updated: 2016/12/4

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

LILRA3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L131T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-LILRA3 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 47
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-LILRA3 antibody (H00011026-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L131T6
    LILRA3 transfected lysate
    Western Blot
    QC Testing of L131T6
    Lane 1: LILRA3 transfected lysate ( 47 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • LILRA3
  • Gene Alias:
  • CD85E,HM31,HM43,ILT6,LIR-4,LIR4,e3
  • Gene Description:
  • leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 3
  • Gene Summary:
  • Leukocyte Ig-like receptors (LIRs) are a family of immunoreceptors expressed predominantly on monocytes and B cells and at lower levels on dendritic cells and natural killer (NK) cells. All LIRs in subfamily B have an inhibitory function (see, e.g., LILRB1, MIM 604811). LIRs in subfamily A, with short cytoplasmic domains lacking an immunoreceptor tyrosine-based inhibitory motif (ITIM) and with transmembrane regions containing a charged arginine residue, may initiate stimulatory cascades (see LILRA1, MIM 604810). One member of subfamily A (LILRA3) lacks a transmembrane region and is presumed to be a soluble receptor (Borges et al., 1997 [PubMed 9548455]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000067955,OTTHUMP00000067957,leucocyte immunoglobulin-like receptor,leukocyte immunoglobulin-like receptor 4,leukocyte immunoglobulin-like receptor A3
  • RSS
  • YouTube
  • Linkedin
  • Facebook