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LEF1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L130T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-LEF1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 44
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-LEF1 antibody (H00051176-M05) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L130T6
    LEF1 transfected lysate
    Western Blot
    QC Testing of L130T6
    Lane 1: LEF1 transfected lysate ( 44 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • LEF1
  • Gene Alias:
  • DKFZp586H0919,TCF1ALPHA
  • Gene Description:
  • lymphoid enhancer-binding factor 1
  • Gene Summary:
  • LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) (Waterman et al., 1991 [PubMed 2010090]; van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM
  • Other Designations:
  • lymphoid enhancer binding factor-1
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