GLUD2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GLUD2 full length
Host
Human
Theoretical MW (kDa)
61
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GLUD2 antibody (H00002747-M01A) by Western Blots.
SDS-PAGE Gel
GLUD2 transfected lysate
Western Blot
Lane 1: GLUD2 transfected lysate ( 61 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot
Immunoprecipitation
-
Gene Info — GLUD2
Entrez GeneID
2747GeneBank Accession#
BC005111.1Protein Accession#
AAH05111.1Gene Name
GLUD2
Gene Alias
GDH2, GLUDP1
Gene Description
glutamate dehydrogenase 2
Omim ID
300144Gene Ontology
HyperlinkGene Summary
Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and/or NADP as cofactors. See also GLUD1 (MIM 138130).[supplied by OMIM
Other Designations
glutamate dehydrogenase pseudogene 1
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com