Transient overexpression cell lysate was tested with Anti-HMGB1 antibody (H00003146-M01) by Western Blots. SDS-PAGE Gel
HMGB1 transfected lysate Western Blot
Lane 1: HMGB1 transfected lysate ( 25 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Amphoterin,OTTHUMP00000018199,OTTHUMP00000190860,Sulfoglucuronyl carbohydrate binding protein,high mobility group box 1,high mobility group protein 1,high-mobility group (nonhistone chromosomal) protein 1