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HLA-DPB1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L127T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-HLA-DPB1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 29
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HLA-DPB1 antibody (H00003115-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L127T6
    HLA-DPB1 transfected lysate
    Western Blot
    QC Testing of L127T6
    Lane 1: HLA-DPB1 transfected lysate ( 29 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3115
  • Gene Name:
  • HLA-DPB1
  • Gene Alias:
  • DPB1,HLA-DP1B
  • Gene Description:
  • major histocompatibility complex, class II, DP beta 1
  • Gene Summary:
  • HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq
  • Other Designations:
  • HLA DP14-beta chain,HLA-DP histocompatibility type, beta-1 subunit,MHC HLA DPB1,MHC class II HLA-DP-beta,MHC class II HLA-DP-beta-1,MHC class II HLA-DRB1,MHC class II antigen DP beta 1 chain,MHC class II antigen DPbeta1,MHC class II antigen beta chain,OTT
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