HAGH HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-HAGH full length
Host
Human
Theoretical MW (kDa)
29
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HAGH antibody (H00003029-M02A) by Western Blots.
SDS-PAGE Gel
HAGH transfected lysate
Western Blot
Lane 1: HAGH transfected lysate ( 29 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — HAGH
Entrez GeneID
3029GeneBank Accession#
NM_005326Protein Accession#
NP_005317Gene Name
HAGH
Gene Alias
GLO2, GLX2, GLXII, HAGH1
Gene Description
hydroxyacylglutathione hydrolase
Omim ID
138760Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. [provided by RefSeq
Other Designations
glyoxalase II|hydroxyacyl glutathione hydrolase|hydroxyacylglutathione hydroxylase
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