Products

Transfected Cell Line

HEK293

Plasmid

pCMV-H3F3B full length

Host

Human

Theoretical MW (kDa)

15

Lysis Buffer

Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Concentration

2 mg/ml

Quality Control Testing

Transient overexpression cell lysate was tested with Anti-H3F3B antibody (H00003021-M01) by Western Blots.

SDS-PAGE Gel

H3F3B transfected lysate

Western Blot

Lane 1: H3F3B transfected lysate ( 15 KDa).
Lane 2: Non-transfected lysate.

Recommend Usage

Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

Storage Buffer

In modified RIPA Lysis Buffer.

Storage Instruction

Store at -80°C. Aliquot to avoid repeated freezing and thawing.