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GTF2IRD1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L121T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GTF2IRD1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 106
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 1.9 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GTF2IRD1 antibody (H00009569-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L121T6
    GTF2IRD1 transfected lysate
    Western Blot
    QC Testing of L121T6
    Lane 1: GTF2IRD1 transfected lysate ( 106 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 9569
  • Gene Name:
  • GTF2IRD1
  • Gene Alias:
  • BEN,CREAM1,GTF3,MUSTRD1,RBAP2,WBS,WBSCR11,WBSCR12,hMusTRD1alpha1
  • Gene Description:
  • GTF2I repeat domain containing 1
  • Gene Summary:
  • The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing of this gene generates at least 2 transcript variants. [provided by RefSeq
  • Other Designations:
  • GTF2I repeat domain-containing 1,Williams-Beuren syndrome chromosome region 11,binding factor for early enhancer,general transcription factor 3,muscle TFII-I repeat domain-containing protein 1 alpha 1
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