GTF2IRD1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GTF2IRD1 full length
Host
Human
Theoretical MW (kDa)
106
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
1.9 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GTF2IRD1 antibody (H00009569-M01) by Western Blots.
SDS-PAGE Gel
GTF2IRD1 transfected lysate
Western Blot
Lane 1: GTF2IRD1 transfected lysate ( 106 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GTF2IRD1
Entrez GeneID
9569GeneBank Accession#
NM_005685Protein Accession#
NP_005676Gene Name
GTF2IRD1
Gene Alias
BEN, CREAM1, GTF3, MUSTRD1, RBAP2, WBS, WBSCR11, WBSCR12, hMusTRD1alpha1
Gene Description
GTF2I repeat domain containing 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing of this gene generates at least 2 transcript variants. [provided by RefSeq
Other Designations
GTF2I repeat domain-containing 1|Williams-Beuren syndrome chromosome region 11|binding factor for early enhancer|general transcription factor 3|muscle TFII-I repeat domain-containing protein 1 alpha 1
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Interactome
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Pathway
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Disease
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