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GRAP2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L119T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GRAP2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 38
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GRAP2 antibody (H00009402-M01) by Western Blots.
    Western Blot
    QC Testing of L119T6
    Lane 1: GRAP2 transfected lysate ( 38 KDa).
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of L119T6
    GRAP2 transfected lysate
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 9402
  • Gene Name:
  • GRAP2
  • Gene Alias:
  • Gene Description:
  • GRB2-related adaptor protein 2
  • Gene Summary:
  • This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq
  • Other Designations:
  • GRB-2-like protein,GRB2-related protein with insert domain,OTTHUMP00000028837,SH3-SH2-SH3 adaptor molecule,growth factor receptor-binding protein,growth factor receptor-bound protein 2-related adaptor protein 2,hematopoietic cell-associated adaptor protei
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