Transient overexpression cell lysate was tested with Anti-GRAP2 antibody (H00009402-M01) by Western Blots. Western Blot
Lane 1: GRAP2 transfected lysate ( 38 KDa). Lane 2: Non-transfected lysate. SDS-PAGE Gel
GRAP2 transfected lysate
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq
GRB-2-like protein,GRB2-related protein with insert domain,OTTHUMP00000028837,SH3-SH2-SH3 adaptor molecule,growth factor receptor-binding protein,growth factor receptor-bound protein 2-related adaptor protein 2,hematopoietic cell-associated adaptor protei