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GPD2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L117T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GPD2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 41
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GPD2 antibody (H00002820-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L117T6
    GPD2 transfected lysate
    Western Blot
    QC Testing of L117T6
    Lane 1: GPD2 transfected lysate ( 41 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2820
  • Gene Name:
  • GPD2
  • Gene Alias:
  • Gene Description:
  • glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
  • Gene Summary:
  • Mitochondrial glycerophosphate dehydrogenase (EC, or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM
  • Other Designations:
  • mitochondrial glycerophosphate dehydrogenase
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