NFATC21P HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FLJ14639 full length
Host
Human
Theoretical MW (kDa)
15
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FLJ14639 antibody (H00084901-M01) by Western Blots.
SDS-PAGE Gel
NFATC2IP transfected lysate
Western Blot
Lane 1: NFATC2IP transfected lysate ( 15 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — NFATC2IP
Entrez GeneID
84901GeneBank Accession#
BC018311Protein Accession#
AAH18311Gene Name
NFATC2IP
Gene Alias
FLJ14639, MGC126790, MGC138387
Gene Description
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein
Gene Ontology
HyperlinkGene Summary
cytoplasmic
Other Designations
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