LSM16 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FLJ21128 full length
Host
Human
Theoretical MW (kDa)
56
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FLJ21128 antibody (H00080153-M01) by Western Blots.
SDS-PAGE Gel
LSM16 transfected lysate
Western Blot
Lane 1: LSM16 transfected lysate ( 56 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot
Immunoprecipitation
-
Gene Info — EDC3
Entrez GeneID
80153GeneBank Accession#
BC011534Protein Accession#
AAH11534Gene Name
EDC3
Gene Alias
FLJ21128, FLJ31777, LSM16, YJDC, YJEFN2
Gene Description
enhancer of mRNA decapping 3 homolog (S. cerevisiae)
Omim ID
609842Gene Ontology
HyperlinkGene Summary
EDC3 is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM
Other Designations
LSM16 homolog (EDC3, S. cerevisiae)|enhancer of mRNA decapping 3|hYjeF_N2-15q23|yjeF domain containing
-
Interactome
-
Pathway
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com