Transient overexpression cell lysate was tested with Anti-FLJ21128 antibody (H00080153-M01) by Western Blots. SDS-PAGE Gel
LSM16 transfected lysate Western Blot
Lane 1: LSM16 transfected lysate ( 56 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
EDC3 is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM
LSM16 homolog (EDC3, S. cerevisiae),enhancer of mRNA decapping 3,hYjeF_N2-15q23,yjeF domain containing