FRG1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FRG1 full length
Host
Human
Theoretical MW (kDa)
29
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FRG1 antibody (H00002483-M01) by Western Blots.
SDS-PAGE Gel
FRG1 transfected lysate
Western Blot
Lane 1: FRG1 transfected lysate ( 29 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FRG1
Entrez GeneID
2483GeneBank Accession#
BC053997Protein Accession#
AAH53997Gene Name
FRG1
Gene Alias
FRG1A, FSG1
Gene Description
FSHD region gene 1
Omim ID
601278Gene Ontology
HyperlinkGene Summary
This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq
Other Designations
facioscapulohumeral muscular dystrophy region gene-1
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