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FXR1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L107T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-FXR1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 61
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-FXR1 antibody (H00008087-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L107T6
    FXR1 transfected lysate
    Western Blot
    QC Testing of L107T6
    Lane 1: FXR1 transfected lysate ( 61 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 8087
  • Gene Name:
  • FXR1
  • Gene Alias:
  • -
  • Gene Description:
  • fragile X mental retardation, autosomal homolog 1
  • Gene Summary:
  • The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • fragile X mental retardation-related protein 1
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