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FYN HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L106T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-FYN full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 55
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-FYN antibody (H00002534-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L106T6
    FYN transfected lysate
    Western Blot
    QC Testing of L106T6
    Lane 1: FYN transfected lysate ( 55 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2534
  • Gene Name:
  • FYN
  • Gene Alias:
  • MGC45350,SLK,SYN
  • Gene Description:
  • FYN oncogene related to SRC, FGR, YES
  • Gene Summary:
  • This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq
  • Other Designations:
  • OKT3-induced calcium influx regulator,OTTHUMP00000017914,OTTHUMP00000017915,OTTHUMP00000017917,c-syn protooncogene,protein-tyrosine kinase fyn,proto-oncogene tyrosine-protein kinase fyn,src-like kinase,src/yes-related novel,tyrosine kinase p59fyn(T)
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