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G22P1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L105T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-G22P1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 70
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-G22P1 antibody (H00002547-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L105T6
    XRCC6 transfected lysate
    Western Blot
    QC Testing of L105T6
    Lane 1: XRCC6 transfected lysate ( 70 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2547
  • Gene Name:
  • XRCC6
  • Gene Alias:
  • CTC75,CTCBF,G22P1,KU70,ML8,TLAA
  • Gene Description:
  • X-ray repair complementing defective repair in Chinese hamster cells 6
  • Gene Summary:
  • The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq
  • Other Designations:
  • ATP-dependent DNA helicase II, 70 kDa subunit,CTC box binding factor 75 kDa subunit,Ku autoantigen p70 subunit,Ku autoantigen, 70kDa,OTTHUMP00000028581,thyroid autoantigen 70kD (Ku antigen),thyroid autoantigen 70kDa (Ku antigen),thyroid-lupus autoantigen
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