GABBR1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
- More Functions
SDS-PAGE Gel
GABBR1 transfected lysate
Western Blot
Lane 1: GABBR1 transfected lysate ( 95 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GABBR1 full length
Host
Human
Theoretical MW (kDa)
95
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GABBR1 antibody (H00002550-M01) by Western Blots.
SDS-PAGE Gel
GABBR1 transfected lysate
Western Blot
Lane 1: GABBR1 transfected lysate ( 95 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GABBR1
Entrez GeneID
2550GeneBank Accession#
BC050532Protein Accession#
AAH50532Gene Name
GABBR1
Gene Alias
FLJ92613, GABAB(1e), GABABR1, GABBR1-3, GPRC3A, dJ271M21.1.1, dJ271M21.1.2, hGB1a
Gene Description
gamma-aminobutyric acid (GABA) B receptor, 1
Omim ID
603540Gene Ontology
HyperlinkGene Summary
Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq
Other Designations
GABA-B receptor|GABAB, subunit 1c|OTTHUMP00000029114|OTTHUMP00000029115|OTTHUMP00000029116|OTTHUMP00000109099|gamma-aminobutyric acid (GABA) B receptor 1|seven transmembrane helix receptor
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Interactome
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Pathway
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Disease
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