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GABBR1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L104T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GABBR1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 95
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GABBR1 antibody (H00002550-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L104T6
    GABBR1 transfected lysate
    Western Blot
    QC Testing of L104T6
    Lane 1: GABBR1 transfected lysate ( 95 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2550
  • Gene Name:
  • GABBR1
  • Gene Alias:
  • FLJ92613,GABAB(1e),GABABR1,GABBR1-3,GPRC3A,dJ271M21.1.1,dJ271M21.1.2,hGB1a
  • Gene Description:
  • gamma-aminobutyric acid (GABA) B receptor, 1
  • Gene Summary:
  • Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • GABA-B receptor,GABAB, subunit 1c,OTTHUMP00000029114,OTTHUMP00000029115,OTTHUMP00000029116,OTTHUMP00000109099,gamma-aminobutyric acid (GABA) B receptor 1,seven transmembrane helix receptor
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