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GALT HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L102T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GALT full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 43
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GALT antibody (H00002592-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L102T6
    GALT transfected lysate
    Western Blot
    QC Testing of L102T6
    Lane 1: GALT transfected lysate ( 43 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2592
  • Gene Name:
  • GALT
  • Gene Alias:
  • -
  • Gene Description:
  • galactose-1-phosphate uridylyltransferase
  • Gene Summary:
  • Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000021298,galactose-1-phosphate uridyl transferase
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