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GART HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L101T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GART full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 46
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GART antibody (H00002618-M01) by Western Blots.
    Western Blot
    QC Testing of L101T6
    Lane 1: GART transfected lysate ( 46 KDa).
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of L101T6
    GART transfected lysate
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2618
  • Gene Name:
  • GART
  • Gene Alias:
  • AIRS,GARS,GARTF,MGC47764,PAIS,PGFT,PRGS
  • Gene Description:
  • phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
  • Gene Summary:
  • The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000068280,OTTHUMP00000068281,OTTHUMP00000068282,OTTHUMP00000068337,trifunctional purine biosynthetic protein adenosine-3
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