GART HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GART full length
Host
Human
Theoretical MW (kDa)
46
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GART antibody (H00002618-M01) by Western Blots.
SDS-PAGE Gel
GART transfected lysate
Western Blot
Lane 1: GART transfected lysate ( 46 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GART
Entrez GeneID
2618GeneBank Accession#
BC038958Protein Accession#
AAH38958Gene Name
GART
Gene Alias
AIRS, GARS, GARTF, MGC47764, PAIS, PGFT, PRGS
Gene Description
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Omim ID
138440Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000068280|OTTHUMP00000068281|OTTHUMP00000068282|OTTHUMP00000068337|trifunctional purine biosynthetic protein adenosine-3
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Interactome
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Pathway
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Disease
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