GFAP HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GFAP full length
Host
Human
Theoretical MW (kDa)
50
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GFAP antibody (H00002670-M01) by Western Blots.
SDS-PAGE Gel
GFAP transfected lysate
Western Blot
Lane 1: GFAP transfected lysate ( 50 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GFAP
Entrez GeneID
2670GeneBank Accession#
BC041765Protein Accession#
AAH41765Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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