Transient overexpression cell lysate was tested with Anti-GFI1 antibody (H00002672-M01) by Western Blots. SDS-PAGE Gel
GFI1 transfected lysate Western Blot
Lane 1: GFI1 transfected lysate ( 45 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq
OTTHUMP00000012582,OTTHUMP00000012583,growth factor independence-1,growth factor independent 1,zinc finger protein 163,zinc finger protein Gfi-1