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GJB3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L097T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-GJB3 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 31
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GJB3 antibody (H00002707-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L097T6
    GJB3 transfected lysate
    Western Blot
    QC Testing of L097T6
    Lane 1: GJB3 transfected lysate ( 31 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2707
  • Gene Name:
  • GJB3
  • Gene Alias:
  • CX31,DFNA2,EKV,FLJ22486,MGC102938
  • Gene Description:
  • gap junction protein, beta 3, 31kDa
  • Gene Summary:
  • This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000004183,OTTHUMP00000004184,connexin 31
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