GJB4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GJB4 full length
Host
Human
Theoretical MW (kDa)
30
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GJB4 antibody (H00127534-M01) by Western Blots.
SDS-PAGE Gel
GJB4 transfected lysate
Western Blot
Lane 1: GJB4 transfected lysate ( 30 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GJB4
Entrez GeneID
127534GeneBank Accession#
BC034709Protein Accession#
AAH34709Gene Name
GJB4
Gene Alias
CX30.3, EKV, MGC21116
Gene Description
gap junction protein, beta 4, 30.3kDa
Gene Ontology
HyperlinkGene Summary
beta 4
Other Designations
OTTHUMP00000004185|connexin 30.3|gap junction protein, beta 4|gap junction protein, beta 4 (connexin 30.3)
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