GKN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GKN1 full length
Host
Human
Theoretical MW (kDa)
20
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GKN1 antibody (H00056287-M01) by Western Blots.
SDS-PAGE Gel
GKN1 transfected lysate
Western Blot
Lane 1: GKN1 transfected lysate ( 20 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GKN1
Entrez GeneID
56287GeneBank Accession#
BC059778Protein Accession#
AAH59778Gene Name
GKN1
Gene Alias
AMP18, BRICD1, CA11, FOV, MGC70354, foveolin
Gene Description
gastrokine 1
Omim ID
606402Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. [provided by RefSeq
Other Designations
18 kDa antrum mucosa protein|BRICHOS domain containing 1
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