FCGR2B HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FCGR2B full length
Host
Human
Theoretical MW (kDa)
34
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FCGR2B antibody (H00002213-M01) by Western Blots.
SDS-PAGE Gel
FCGR2B transfected lysate
Western Blot
Lane 1: FCGR2B transfected lysate ( 34 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FCGR2B
Entrez GeneID
2213GeneBank Accession#
BC031992Protein Accession#
AAH31992Gene Name
FCGR2B
Gene Alias
CD32, CD32B, FCG2, FCGR2, IGFR2
Gene Description
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Gene Ontology
HyperlinkGene Summary
low affinity IIb
Other Designations
Fc fragment of IgG, low affinity II, receptor for (CD32)|Fc fragment of IgG, low affinity IIb, receptor for (CD32)|Fc gamma RIIb|OTTHUMP00000032379
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