Transient overexpression cell lysate was tested with Anti-FBLP-1 antibody (H00054751-M10) by Western Blots. SDS-PAGE Gel
FBLIM1 transfected lysate Western Blot
Lane 1: FBLIM1 transfected lysate ( 41 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
CSX-associated LIM,MIG2-interacting protein,OTTHUMP00000003118,OTTHUMP00000003119,OTTHUMP00000003120,filamin-binding LIM protein-1,migfilin,mitogen-inducible 2 interacting protein