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Last updated: 2016/11/27
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FASN HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L088T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-FASN full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 273
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-FASN antibody (H00002194-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L088T6
    FASN transfected lysate
    Western Blot
    QC Testing of L088T6
    Lane 1: FASN transfected lysate ( 273 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2194
  • Gene Name:
  • FASN
  • Gene Alias:
  • FAS,MGC14367,MGC15706,OA-519,SDR27X1
  • Gene Description:
  • fatty acid synthase
  • Gene Summary:
  • The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq
  • Other Designations:
  • short chain dehydrogenase/reductase family 27X, member 1
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