ETV4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ETV4 full length
Host
Human
Theoretical MW (kDa)
54
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ETV4 antibody (H00002118-M01) by Western Blots.
SDS-PAGE Gel
ETV4 transfected lysate
Western Blot
Lane 1: ETV4 transfected lysate ( 54 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — ETV4
Entrez GeneID
2118GeneBank Accession#
BC007242Protein Accession#
AAH07242Gene Name
ETV4
Gene Alias
E1A-F, E1AF, PEA3, PEAS3
Gene Description
ets variant 4
Omim ID
600711Gene Ontology
HyperlinkGene Summary
E1AF)|ets variant gene 4 (E1A enhancer-binding protein
Other Designations
E1A enhancer binding protein|EWS protein/E1A enhancer binding protein chimera|ets variant gene 4 (E1A enhancer binding protein, E1AF)|ets variant gene 4 (E1A enhancer-binding protein, E1AF)|polyomavirus enhancer activator-3
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