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EPB41 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L084T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-EPB41 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 81
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-EPB41 antibody (H00002035-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L084T6
    EPB41 transfected lysate
    Western Blot
    QC Testing of L084T6
    Lane 1: EPB41 transfected lysate ( 81 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2035
  • Gene Name:
  • EPB41
  • Gene Alias:
  • 4.1R,EL1,HE
  • Gene Description:
  • erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
  • Gene Summary:
  • Elliptocytosis is a hematologic disorder characterized by elliptically shaped erythrocytes and a variable degree of hemolytic anemia. Inherited as an autosomal dominant, elliptocytosis results from mutation in any one of several genes encoding proteins of the red cell membrane skeleton. The form discussed here is the one found in the 1950s to be linked to Rh blood group and more recently shown to be caused by a defect in protein 4.1. 'Rh-unlinked' forms of elliptocytosis are caused by mutation in the alpha-spectrin gene (MIM 182860), the beta-spectrin gene (MIM 182870), or the band 3 gene (MIM 109270).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000003772,OTTHUMP00000003773,OTTHUMP00000003774,erythrocyte surface protein band 4.1
  • Gene Pathway
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