EMP3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-EMP3 full length
Host
Human
Theoretical MW (kDa)
18
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EMP3 antibody (H00002014-M01) by Western Blots.
SDS-PAGE Gel
EMP3 transfected lysate
Western Blot
Lane 1: EMP3 transfected lysate ( 18 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — EMP3
Entrez GeneID
2014GeneBank Accession#
NM_001425.1Protein Accession#
NP_001416Gene Name
EMP3
Gene Alias
YMP
Gene Description
epithelial membrane protein 3
Omim ID
602335Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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