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EEF1G HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L081T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-EEF1G full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 50
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-EEF1G antibody (H00001937-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L081T6
    EEF1G transfected lysate
    Western Blot
    QC Testing of L081T6
    Lane 1: EEF1G transfected lysate ( 50 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1937
  • Gene Name:
  • EEF1G
  • Gene Alias:
  • EF1G,GIG35
  • Gene Description:
  • eukaryotic translation elongation factor 1 gamma
  • Gene Summary:
  • This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq
  • Other Designations:
  • EF-1-gamma,PRO1608,eEF-1B gamma,elongation factor 1-gamma,pancreatic tumor-related protein,translation elongation factor eEF-1 gamma chain
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